| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094513 |
| Chromosome: | chromosome 6 |
| Location: | 8627058 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309150 | ROC56 | (1 of 1) K13220 - WW domain-binding protein 4 (WBP4, FBP21) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTGGACCGCTGCCGCCGCTGCCCTGAG |
| Internal bar code: | TAATGGGTAGGCGTAGATGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 505 |
| LEAP-Seq percent confirming: | 99.1778 |
| LEAP-Seq n confirming: | 1930 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGCAGCAGTAGCAGTTT |
| Suggested primer 2: | GGTACGAGAGAAGCTGTGGC |