| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.094533 |
| Chromosome: | chromosome 17 |
| Location: | 3425011 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724400 | FAP257 | Flagellar Associated Protein 257; (1 of 3) PF05217 - STOP protein (STOP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATACGTGGGTGCTCCGCCGGGTGGCGCGG |
| Internal bar code: | ACTAGATCCCTTTCGACATCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 227 |
| LEAP-Seq percent confirming: | 99.9208 |
| LEAP-Seq n confirming: | 1261 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAATCTCCTGAATCCGGCA |
| Suggested primer 2: | TCACTCCCACGGTGTAATCA |