Insertion junction: LMJ.RY0402.094535_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.094535
Chromosome:chromosome 3
Location:3064480
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g164450 (1 of 100) IPR001810 - F-box domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTGCCCAGGGCACCCTTCTGCCTCTGCT
Internal bar code:TCCGAGAGTCAGCGCCTGAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:92
LEAP-Seq percent confirming:98.1308
LEAP-Seq n confirming:105
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGAACCCACTCCACCAACAG
Suggested primer 2:CGAACCGACATACACACAGG