Insertion junction: LMJ.RY0402.094569_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.094569
Chromosome:chromosome_12
Location:9559903
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g546877 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CATGGCGGCGGCCGGTGTGGCGCGGCAGGC
Internal bar code:TGAGTAGATTGGCAGGGCGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:19
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCTGACTCTGTTGAAAGC
Suggested primer 2:TAGCTACTGCGCTCATCACC