| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094610 |
| Chromosome: | chromosome 12 |
| Location: | 571845 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492700 | FAS1 | (1 of 10) PTHR10900 - PERIOSTIN-RELATED; Fasciclin-like protein 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCATCTCTGTGCCATGTGCATTATACAT |
| Internal bar code: | GTATATGTTCTCCTCCTAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 81 |
| LEAP-Seq percent confirming: | 99.4667 |
| LEAP-Seq n confirming: | 746 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGCAGGAGGAAGAGGAG |
| Suggested primer 2: | CAGTTGGTAACCCGGAGAAA |