| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.094623 |
| Chromosome: | chromosome_10 |
| Location: | 1931590 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g432050 | FAP284 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGGCTGGCAACGCCCTGAATAAACACGCG |
| Internal bar code: | TTATTTGGGACGACGTGTGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1066 |
| LEAP-Seq percent confirming: | 99.4681 |
| LEAP-Seq n confirming: | 1122 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCACATCCAGTCCACGACA |
| Suggested primer 2: | CAAACTGCGGCAAATCTACA |