Insertion junction: LMJ.RY0402.094638_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.094638
Chromosome:chromosome 12
Location:440753
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g493600 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGAGCCACGCGCCTCGTGCGCTTCCCCCT
Internal bar code:CTTGGACTGTCCGTCCGTGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:44
LEAP-Seq percent confirming:83.3515
LEAP-Seq n confirming:2288
LEAP-Seq n nonconfirming:457
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTGGTGACGGTGTAGCTG
Suggested primer 2:GGCTGGTCGGTCTACAACAT