| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094653 |
| Chromosome: | chromosome 5 |
| Location: | 1835234 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242000 | CHLD,CHLD1 | (1 of 1) K03404 - magnesium chelatase subunit D (chlD, bchD); Magnesium chelatase subunit D, chloroplast precursor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGCGACAAGGCCGAGGTGCTGCTGCCG |
| Internal bar code: | ATGGTCCGTGTCCAGGCAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 87.3684 |
| LEAP-Seq n confirming: | 249 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGCTTGACCTGTCCTCC |
| Suggested primer 2: | AGTACTCCATTCACCGTGCC |