| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094682 |
| Chromosome: | chromosome 16 |
| Location: | 6561747 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672350 | (1 of 1) IPR001357//IPR001841 - BRCT domain // Zinc finger, RING-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTACGCTGGTGCTGGTGGTGTGCGAGAGG |
| Internal bar code: | TAGCCCGCCGTGTAACGAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 322 |
| LEAP-Seq percent confirming: | 99.3399 |
| LEAP-Seq n confirming: | 1204 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATGAAACACTCCCCACTT |
| Suggested primer 2: | TCGAGGTCGTACAATGGTGA |