Insertion junction: LMJ.RY0402.094682_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.094682
Chromosome:chromosome 16
Location:6562501
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g672350 (1 of 1) IPR001357//IPR001841 - BRCT domain // Zinc finger, RING-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCAGCACCACCACCGGCGGGATCGAGAT
Internal bar code:AGCGCTACAATGGACGCGAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:194
LEAP-Seq percent confirming:52.1127
LEAP-Seq n confirming:296
LEAP-Seq n nonconfirming:272
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCTAGCACATCGGTTCAG
Suggested primer 2:ACGGTGCGTGTGTGTAATGT