| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094695 |
| Chromosome: | chromosome 6 |
| Location: | 6452935 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292750 | (1 of 2) PF05694 - 56kDa selenium binding protein (SBP56) (SBP56) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTTCTCCTGTTGGAGGTAAGCGTCGAT |
| Internal bar code: | GCGTGGCAGCCAGGGCTGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 98.7827 |
| LEAP-Seq n confirming: | 2191 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGGTGGTGTGTGTGTGT |
| Suggested primer 2: | CCCGTAATATCCATGCATCC |