| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.094745 |
| Chromosome: | chromosome 6 |
| Location: | 2922884 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272600 | (1 of 1) PF01429 - Methyl-CpG binding domain (MBD) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGGCTTAGAAGCCCGTCCGGGGTCGGG |
| Internal bar code: | TTCGCGAGGAGCTTGTTGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 804 |
| LEAP-Seq percent confirming: | 99.0196 |
| LEAP-Seq n confirming: | 808 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGACAGGCGAGACAACC |
| Suggested primer 2: | ATTGCCAACCCAAGTTTCAG |