Insertion junction: LMJ.RY0402.094763_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.094763
Chromosome:chromosome_10
Location:901645
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre10.g424250 FAP90 Flagellar Associated Protein sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TTGTTTCAGTGCACGTGGCACCACGCATAG
Internal bar code:CGAAAGTCGAGTTCCATTCATA

Confirmation - LEAP-Seq

LEAP-Seq distance:458
LEAP-Seq percent confirming:99.6577
LEAP-Seq n confirming:4658
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTAGAGGCACCTGTAGGA
Suggested primer 2:GATGATGAGCAGGACTGGGT