Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.094763 |
Chromosome: | chromosome_10 |
Location: | 901645 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g424250 | FAP90 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGTTTCAGTGCACGTGGCACCACGCATAG |
Internal bar code: | CGAAAGTCGAGTTCCATTCATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 458 |
LEAP-Seq percent confirming: | 99.6577 |
LEAP-Seq n confirming: | 4658 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGTAGAGGCACCTGTAGGA |
Suggested primer 2: | GATGATGAGCAGGACTGGGT |