| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094764 |
| Chromosome: | chromosome 12 |
| Location: | 9522126 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543477 | (1 of 98) IPR023214 - HAD-like domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGAGTGCGACCGTATGTGTATAGAGACA |
| Internal bar code: | GCACAACTTTTATCAGATCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 849 |
| LEAP-Seq percent confirming: | 92.556 |
| LEAP-Seq n confirming: | 5334 |
| LEAP-Seq n nonconfirming: | 429 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTGGGGAACAACTAGAG |
| Suggested primer 2: | TTTTGTCAGAGCTCCGTCCT |