| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094769 |
| Chromosome: | chromosome 13 |
| Location: | 3529864 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588000 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCATGTCGTGCAGGGCGGCGGTGACGG |
| Internal bar code: | TCGGAACCGCCTCGGATCCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 86.3481 |
| LEAP-Seq n confirming: | 506 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTTCTTCCTTGACGCTG |
| Suggested primer 2: | CCAGTAGCTGTCCCTTCAGC |