| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.094778 |
| Chromosome: | chromosome 13 |
| Location: | 1974260 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576600 | Conserved Protein with Similarity to Catalytic domain of Protein Tyrosine Kinases; (1 of 1) PTHR23257//PTHR23257:SF405 - SERINE-THREONINE PROTEIN KINASE // MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 7-LIKE-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCAGACCATTGACAGGAGTGAGATATCA |
| Internal bar code: | ATGCAGACAGTATGGTCCTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 596 |
| LEAP-Seq percent confirming: | 99.6221 |
| LEAP-Seq n confirming: | 3954 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGTGTGCTCTCTCACCG |
| Suggested primer 2: | TCTCCTCTCCTCCCTGAACA |