| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.094782 |
| Chromosome: | chromosome 9 |
| Location: | 5618901 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400960 | (1 of 1) IPR006311//IPR007657 - Twin-arginine translocation pathway, signal sequence // Glycosyltransferase AER61, uncharacterised | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGTGCGGACCACGGCGACCTGTGGCGCT |
| Internal bar code: | GTGGGTTCTTTAGCCGCCGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 170 |
| LEAP-Seq percent confirming: | 84.6649 |
| LEAP-Seq n confirming: | 657 |
| LEAP-Seq n nonconfirming: | 119 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACGACTGGTTCAAGGAGC |
| Suggested primer 2: | CCACTCGCTTGCTTCTTACC |