| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094788 |
| Chromosome: | chromosome 3 |
| Location: | 4932965 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179860 | (1 of 1) 2.1.1.203 - tRNA (cytosine(34)-C(5))-methyltransferase; tRNA-(m5C) methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTGGTGGCTGTTGAGCTCAAGGCTCGC |
| Internal bar code: | GACCGGCGACGCATAGGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 825 |
| LEAP-Seq percent confirming: | 99.2302 |
| LEAP-Seq n confirming: | 6961 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGTGTGATGGCCTGATG |
| Suggested primer 2: | CTCGCTTCAGACATTAGCCC |