| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.094800 |
| Chromosome: | chromosome 6 |
| Location: | 5140070 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281286 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGCTGTGCTATGGGGGCGCCGAAAGCC |
| Internal bar code: | AGGGGCGATGGAACCGACCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 562 |
| LEAP-Seq percent confirming: | 98.1105 |
| LEAP-Seq n confirming: | 6127 |
| LEAP-Seq n nonconfirming: | 118 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGCCGTGACCTTGTAGA |
| Suggested primer 2: | GGGCTACGCAATCAAGAGAC |