| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094803 |
| Chromosome: | chromosome 12 |
| Location: | 285358 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485500 | (1 of 1) PTHR11757:SF12 - PROLYL ENDOPEPTIDASE-LIKE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGGCAGCAGCAGCCGGCCTGCAGACGG |
| Internal bar code: | GTGTGTATGGCGATCGTGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 146 |
| LEAP-Seq percent confirming: | 54.2865 |
| LEAP-Seq n confirming: | 2077 |
| LEAP-Seq n nonconfirming: | 1749 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGCAGCTCTTGCTGTTG |
| Suggested primer 2: | CCTACACCTACGCCGTCATT |