Insertion junction: LMJ.RY0402.094811_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.094811
Chromosome:chromosome 7
Location:2935009
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g332750 PSL2 Protein of unknown function; (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGAAGCGGCGGCGGGGTGCGTGGGTTGC
Internal bar code:GACCACTTTAGGGTAGTTAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:635
LEAP-Seq percent confirming:92.709
LEAP-Seq n confirming:5633
LEAP-Seq n nonconfirming:443
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTCTCATTTGATCGTGTG
Suggested primer 2:AACAACACCCTGGTACTCGC