| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094827 |
| Chromosome: | chromosome 14 |
| Location: | 4143496 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g634236 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTACGCGTGCACCCCGACGTGGGGCGGT |
| Internal bar code: | CAAGGCAGTCTCGAGCGACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 25 |
| LEAP-Seq percent confirming: | 93.4272 |
| LEAP-Seq n confirming: | 2189 |
| LEAP-Seq n nonconfirming: | 154 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTATCTTAAGGCAGTGGCG |
| Suggested primer 2: | AAATGGGAACTCGTTTGACG |