| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.094833 |
| Chromosome: | chromosome 2 |
| Location: | 5734320 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110263 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGAGGAGTCGATTGACACCACTTTTAT |
| Internal bar code: | CACAAATTCCTATTAGCGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 44.7804 |
| LEAP-Seq n confirming: | 785 |
| LEAP-Seq n nonconfirming: | 968 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGTGGAGCACGTCGATA |
| Suggested primer 2: | AGCCGACAACGCTACCTAGA |