| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094845 |
| Chromosome: | chromosome 6 |
| Location: | 1219864 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257700 | RAD18 | Putative E3 ubiquitin ligase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTCGCCTCCTGCGCCGCCGCTAGCTGC |
| Internal bar code: | AGGTTCCAAAATGGAGGTCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 374 |
| LEAP-Seq percent confirming: | 99.2064 |
| LEAP-Seq n confirming: | 250 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCTCTCCTCTTCCTCCT |
| Suggested primer 2: | GTAGAGTGGCTGCCTCATCC |