Insertion junction: LMJ.RY0402.094846_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.094846
Chromosome:chromosome 17
Location:6300916
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g742700 CGLD30,HLM32 (1 of 1) PTHR22884:SF343 - PROTEIN MET-1, ISOFORM A; SET domain containing protein, putative histone methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTTCACCTTGTCCAGCGTGATCACGATC
Internal bar code:TTTGTTGGTCCGGCGGTTGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:181
LEAP-Seq percent confirming:52.5154
LEAP-Seq n confirming:595
LEAP-Seq n nonconfirming:538
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GACCACATTGATGATGCTCG
Suggested primer 2:GCACCGATATCGTCACAATG