| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094848 |
| Chromosome: | chromosome 14 |
| Location: | 1750782 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619800 | SPO11B,TOP4 | meoisis-related DNA topoisomerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTAAGGAGATGTGGAGGGGAGAGGAGA |
| Internal bar code: | GGTTTTGAGTGTTTGTTAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 576 |
| LEAP-Seq percent confirming: | 96.9128 |
| LEAP-Seq n confirming: | 722 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGTGTGTGCGTGT |
| Suggested primer 2: | GCACATCAACGGTTTGTACG |