| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094851 |
| Chromosome: | chromosome 1 |
| Location: | 3814784 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g024650 | CTL1 | carbohydrate-binding protein; (1 of 2) PF00059//PF07714//PF14295 - Lectin C-type domain (Lectin_C) // Protein tyrosine kinase (Pkinase_Tyr) // PAN domain (PAN_4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCAGCGGTGCTGCTGGTGCTGTTGCTG |
| Internal bar code: | GCGCCATTGGGTGCCTGGCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1214 |
| LEAP-Seq percent confirming: | 99.7505 |
| LEAP-Seq n confirming: | 27185 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACGCTGAATTCCCAGATA |
| Suggested primer 2: | CAAACGTCACATCGGTTTTG |