| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.094861 |
| Chromosome: | chromosome 17 |
| Location: | 1732559 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708950 | (1 of 2) 3.2.1.58 - Glucan 1,3-beta-glucosidase / Exo-1,3-beta-glucosidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTCAGTCCTTGCGCGAGGCCGATAATC |
| Internal bar code: | AAAAGTGTGAGCGATCAGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 98.9247 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTGGTTATCCCTTCATA |
| Suggested primer 2: | AGGTCATTGGGAAGAACGTG |