| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094865 |
| Chromosome: | chromosome 7 |
| Location: | 480599 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315650 | SMM23 | (1 of 1) 2.1.1.164 - Demethylrebeccamycin-D-glucose O-methyltransferase; S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGGTGGTTCAGAGCTCCGGCCCCCCCCC |
| Internal bar code: | CGTGTCACCGGTACGGTAAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 365 |
| LEAP-Seq percent confirming: | 99.3259 |
| LEAP-Seq n confirming: | 4715 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTGCCGAGATGGATTAT |
| Suggested primer 2: | CACTCACAGCAGCTCCACAT |