| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094876 |
| Chromosome: | chromosome 7 |
| Location: | 6025225 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354800 | (1 of 2) K11145 - ribonuclease III family protein [EC:3.1.26.-] (K11145) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCGAGAGGTGTGAGGGCACTGCGCAAC |
| Internal bar code: | TCTGCGGGGCTGCCCGCTAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1178 |
| LEAP-Seq percent confirming: | 99.3567 |
| LEAP-Seq n confirming: | 12511 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTTTGAAGCCAGCCTAC |
| Suggested primer 2: | CAGCAGCAGACGGAAATGTA |