| Insertion cassette: | CIB1 | 
| Side of cassette: | 5' | 
| Strand: | - | 
| Strain: | LMJ.RY0402.094947 | 
| Chromosome: | chromosome 2 | 
| Location: | 4339279 | 
| Confidence (%): | 95 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre02.g099251 | (1 of 7) PF02517 - CAAX protease self-immunity (Abi) | CDS | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAACTACAGCTACAGCGTGGCAGCGATG | 
| Internal bar code: | GAGCGTGGTGGAAGCGGTCGTC | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 | 
| LEAP-Seq percent confirming: | 99.8399 | 
| LEAP-Seq n confirming: | 12475 | 
| LEAP-Seq n nonconfirming: | 20 | 
| LEAP-Seq n unique pos: | 5 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCTTCTTCAGCATCTCC | 
| Suggested primer 2: | CTCACCCAACTCCCTTGTGT |