Insertion junction: LMJ.RY0402.094949_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.094949
Chromosome:chromosome 6
Location:5399286
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g283900 (1 of 1) PTHR16007//PTHR16007:SF15 - EPIDIDYMAL MEMBRANE PROTEIN E9-RELATED // TRANSMEMBRANE PROTEIN 45B intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCAACTGCCAGCGTAGGTGCACGCAAGCA
Internal bar code:CGAGGCTCGGGACAACAAAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:687
LEAP-Seq percent confirming:99.5025
LEAP-Seq n confirming:400
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AACAGGTTGAGGGTGGAATG
Suggested primer 2:CAAAGCGCATTTGCTGAATA