Insertion junction: LMJ.RY0402.094987_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.094987
Chromosome:chromosome 6
Location:1015986
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g256050 (1 of 19) 3.6.1.3 - Adenosinetriphosphatase / Triphosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTTGCGCCGCCAGCTGCTGGGGATGCCG
Internal bar code:TATGGTGTCGCAAATATAAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:903
LEAP-Seq percent confirming:99.1254
LEAP-Seq n confirming:680
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTCACTCCCGTTCATGTGTG
Suggested primer 2:TCACATCCGTGTATGGCACT