| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.095030 |
| Chromosome: | chromosome 12 |
| Location: | 2040218 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509950 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATTCATTACAGCATCATCGACAGTTGA |
| Internal bar code: | CGTACGACGACTGCGCGGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 669 |
| LEAP-Seq percent confirming: | 99.0893 |
| LEAP-Seq n confirming: | 3373 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAACCTCCTGAACCCACCC |
| Suggested primer 2: | CCGGATCCGCTATGTTTTTA |