Insertion junction: LMJ.RY0402.095088_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.095088
Chromosome:chromosome 2
Location:7304247
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g145452 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGACTCTTGCGCTACTGGCGACTGGACC
Internal bar code:TTCGTTGGACAGTGTAACCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:306
LEAP-Seq percent confirming:99.4025
LEAP-Seq n confirming:1830
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CATATGACAGCCGCAGCTAA
Suggested primer 2:AGCAGGGATGTACGGTTGAC