| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.095108 |
| Chromosome: | chromosome 12 |
| Location: | 2511657 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506350 | HFO18 | (1 of 33) K11254 - histone H4 (H4); Histone H4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACGTATCAGCGGCCTCATCTACGAGGA |
| Internal bar code: | CAGGGGGGTCTTTCCGGAGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 517 |
| LEAP-Seq percent confirming: | 98.8745 |
| LEAP-Seq n confirming: | 1757 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATCTTGGACAGGTGATT |
| Suggested primer 2: | GTGATGGAGTGGATTTGGCT |