| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.095126 |
| Chromosome: | chromosome_2 |
| Location: | 1054176 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g080700 | BIP1 | Endoplasmic reticulum associated Hsp70 protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCCCAGACCCCAGCAGATGACAACCAATT |
| Internal bar code: | CTGGGCGGGAGTTCGAGGCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 91.2534 |
| LEAP-Seq n confirming: | 1325 |
| LEAP-Seq n nonconfirming: | 127 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAACACCCCTCATCTCGT |
| Suggested primer 2: | AGGTTTGAATGGTCAGTCGG |