Insertion junction: LMJ.RY0402.095128_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.095128
Chromosome:chromosome 6
Location:5543985
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g285150 APx-R,APX2 Ascorbate peroxidase-related; (1 of 3) 1.11.1.11 - L-ascorbate peroxidase / Ascorbic acid peroxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGCACGCTCGTCGCGGGTTGCCATGGCG
Internal bar code:TAGCCGCACCGCTAGTATGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:546
LEAP-Seq percent confirming:98.9121
LEAP-Seq n confirming:1182
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGTAACTCGCACCCTCTC
Suggested primer 2:ACCAGTTTGGGGTTGTGGTA