| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.095138 |
| Chromosome: | chromosome 7 |
| Location: | 4741944 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g345300 | UAP1 | UDP-N-acetylglucosamine-pyrophosphorylase-related protein; (1 of 1) K00972 - UDP-N-acetylglucosamine/UDP-N-acetylgalactosamine diphosphorylase (UAP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACACGCCTACGCCGCTGGCCGCTGCAG |
| Internal bar code: | ATCTCTGTGTTTAAGTATTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 293 |
| LEAP-Seq percent confirming: | 99.7082 |
| LEAP-Seq n confirming: | 1025 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACCGTGTCTGTGTGTACG |
| Suggested primer 2: | AATACAAACCTTTGCGGCTG |