| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095161 |
| Chromosome: | chromosome 16 |
| Location: | 2171201 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658200 | (1 of 2) IPR003594//IPR024975 - Histidine kinase-like ATPase, C-terminal domain // Domain of unknown function DUF3883 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATTCCGCGGCACTCCCCAACGACACCC |
| Internal bar code: | GGGTTCGGACAGTTGTGCCTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 158 |
| LEAP-Seq percent confirming: | 8.18521 |
| LEAP-Seq n confirming: | 373 |
| LEAP-Seq n nonconfirming: | 4184 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCACCCCTGCTGTATTTCC |
| Suggested primer 2: | TTTCTCTGGAGACCCCAATG |