| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095163 |
| Chromosome: | chromosome 2 |
| Location: | 919620 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079600 | TSF2 | (1 of 1) PTHR11538:SF41 - PHENYLALANINE--TRNA LIGASE, MITOCHONDRIAL; Phenylalanyl-tRNA synthetase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTCCCGATGCAGTCGCGATTCCTGTTG |
| Internal bar code: | TCGGGTTTACCCTTTGTCCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 502 |
| LEAP-Seq percent confirming: | 98.9813 |
| LEAP-Seq n confirming: | 583 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTTTGCTGGTAGGTGTG |
| Suggested primer 2: | CACGGGGTAGTGAGTGGAGT |