| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.095171 |
| Chromosome: | chromosome_8 |
| Location: | 1038962 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g362100 | FAL11 | Similar to Flagellar Associated Protein FAP154 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGACAATCTTACCAATGGTGCCTGCTCCA |
| Internal bar code: | GTGGGGGCTTAAGAACTCACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 99.5317 |
| LEAP-Seq n confirming: | 3826 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAATATCCCACCCTCCT |
| Suggested primer 2: | AGGCCAAACTCGCTCACTAA |