Insertion junction: LMJ.RY0402.095178_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.095178
Chromosome:chromosome 17
Location:2701516
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g717950 PHC28,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 6 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTATCCATCTCAAACGTATTTGAAGCGCC
Internal bar code:ACGGGCCGGCGAATATCGTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:692
LEAP-Seq percent confirming:99.1914
LEAP-Seq n confirming:368
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCAACAATGTAAGCTGAA
Suggested primer 2:CGAGGTACCACGGAAGTCAT