| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.095178 |
| Chromosome: | chromosome_17 |
| Location: | 2701516 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g717950 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTATCCATCTCAAACGTATTTGAAGCGCC |
| Internal bar code: | ACGGGCCGGCGAATATCGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 692 |
| LEAP-Seq percent confirming: | 99.1914 |
| LEAP-Seq n confirming: | 368 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCAACAATGTAAGCTGAA |
| Suggested primer 2: | CGAGGTACCACGGAAGTCAT |