| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.095184 |
| Chromosome: | chromosome 1 |
| Location: | 111687 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000800 | CGL136 | Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTCCGGGTGGCGCACTCGCGCGCACTCT |
| Internal bar code: | ACATGGCTCGATTTGGTGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 615 |
| LEAP-Seq percent confirming: | 90.5632 |
| LEAP-Seq n confirming: | 1190 |
| LEAP-Seq n nonconfirming: | 124 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACGAAAACCTTCCTGAAA |
| Suggested primer 2: | GTTTGACACGGCATTGTGAC |