| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.095187 |
| Chromosome: | chromosome 7 |
| Location: | 2714963 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331114 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAACAATGCAACGAGAGCGCCGATTGAGT |
| Internal bar code: | TTGCTAGACATAGAGGTGCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 958 |
| LEAP-Seq percent confirming: | 99.602 |
| LEAP-Seq n confirming: | 1001 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGTGACAGCATTGCAT |
| Suggested primer 2: | AGCGAGGTGGTGGATACAAG |