| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095189 |
| Chromosome: | chromosome 3 |
| Location: | 4531815 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176550 | PRMT5,PRM5 | (1 of 1) K02516 - protein arginine N-methyltransferase 5 (PRMT5, HSL7); Protein-/Histone-arginine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCCTGGAACAGACTACCCAACCCACCC |
| Internal bar code: | GGATTTTATCCTTGTGTGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 78.7942 |
| LEAP-Seq n confirming: | 379 |
| LEAP-Seq n nonconfirming: | 102 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGCTTTTCCAAACATCC |
| Suggested primer 2: | ACACACACACACACACACGG |