| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.095204 |
| Chromosome: | chromosome 3 |
| Location: | 5812657 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189050 | EMAN,EAM1 | (1 of 1) 3.2.1.130 - Glycoprotein endo-alpha-1,2-mannosidase; Endomannosidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACCGGTTATGGGTACAGATGGTTGGGG |
| Internal bar code: | GCCCCACAGAGTGACGTGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 521 |
| LEAP-Seq percent confirming: | 99.0398 |
| LEAP-Seq n confirming: | 5570 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTTTGACGGCTTCTACA |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |