| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.095212 |
| Chromosome: | chromosome 16 |
| Location: | 722062 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691202 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGCAGACGTGGTCGGGCTGCTCCCGTGC |
| Internal bar code: | GACCGGCCTGGCCGGCGGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 97.7573 |
| LEAP-Seq n confirming: | 2223 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGACGAGGAGTTCAGAGC |
| Suggested primer 2: | TTACTGACAGGGGTTTTCCG |