Insertion junction: LMJ.RY0402.095221_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.095221
Chromosome:chromosome 14
Location:2220195
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g622901 (1 of 21) IPR001611//IPR003591 - Leucine-rich repeat // Leucine-rich repeat, typical subtype CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGGCTCCCCCAGCACGCGCAACAGCTG
Internal bar code:TTCGATGTTCTTCACTGTGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:291
LEAP-Seq percent confirming:78.9474
LEAP-Seq n confirming:150
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCTAGCTCGAGTCTGCTG
Suggested primer 2:AGGGCTTGGGGTATCGTAGT