Insertion junction: LMJ.RY0402.095229_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.095229
Chromosome:chromosome 17
Location:3575523
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g725600 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGCGCCCGTGAGGCCCGCGGCGGGCGG
Internal bar code:CGATGTGCCGCTCGAACCATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:320
LEAP-Seq percent confirming:99.3743
LEAP-Seq n confirming:1747
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTCCCTCGTGTCTCTCTC
Suggested primer 2:ATCACCAAACCAGACCGAAG